EGAS00001005376 1M-scBloodNL

Here we present the 1M-scBloodNL study, in which we performed single-cell RNA-seq on 120 individuals of the Northern Netherlands population cohort Lifelines. For each individual peripheral blood mononuclear cells (PBMCs) were sequenced in an unstimulated condition, and after 3 and 24 hour in vitro stimulation with C. albicans (CA), M. tuberculosis (MTB) and P. aeruginosa (PA), totalling approximately 1.3 million cells. scRNA-seq was conducted with the 10X Genomics 3'-end v2 (72 libraries) and v3 (33 libraries) technology. In general, each library contains PBMCs from 8 donors and 2 different stimulation-timepoint combinations. Donors were demultiplexed using a combination of SoupOrCell (https://www.nature.com/articles/s41592-020-0820-1) and genotype information to assign the correct donor to a donor-specific cell cluster.

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Title EGAS00001005376 1M-scBloodNL
Description

Here we present the 1M-scBloodNL study, in which we performed single-cell RNA-seq on 120 individuals of the Northern Netherlands population cohort Lifelines. For each individual peripheral blood mononuclear cells (PBMCs) were sequenced in an unstimulated condition, and after 3 and 24 hour in vitro stimulation with C. albicans (CA), M. tuberculosis (MTB) and P. aeruginosa (PA), totalling approximately 1.3 million cells. scRNA-seq was conducted with the 10X Genomics 3'-end v2 (72 libraries) and v3 (33 libraries) technology. In general, each library contains PBMCs from 8 donors and 2 different stimulation-timepoint combinations. Donors were demultiplexed using a combination of SoupOrCell (https://www.nature.com/articles/s41592-020-0820-1) and genotype information to assign the correct donor to a donor-specific cell cluster.
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Contact points
Contact point 1
URI
http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/Contacts/firstName=Monique%20van%20der&lastName=Wijst&resource=EGAS00001005376
Name
Monique van der Wijst
Name (translations)
Email
m.g.p.van.der.wijst@umcg.nl
Identifier
URL
Publisher
Publisher 1
URI
http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/Agents/id=UMCG&resource=EGAS00001005376
Name
University Medical Center Groningen
Name (translations)
Email
researchdatacatalogue@umcg.nl
URL
https://www.umcg.nl/
Type
Identifier
https://ror.org/03cv38k47
Creator
Creator 1
URI
http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/Agents/id=UMCG&resource=EGAS00001005376
Name
University Medical Center Groningen
Name (translations)
Email
researchdatacatalogue@umcg.nl
URL
https://www.umcg.nl/
Type
Identifier
https://ror.org/03cv38k47
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    Identifier https://ega-archive.org/datasets/EGAD00001007764
    Frequency
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    Temporal coverage
    Temporal resolution
    Spatial coverage
    Spatial resolution in meters
    Access rights http://publications.europa.eu/resource/authority/access-right/NON_PUBLIC
    Other identifier
    Theme
    1. http://publications.europa.eu/resource/authority/data-theme/HEAL
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    Applicable legislation
    1. http://data.europa.eu/eli/reg/2022/868/oj
    Has version
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    Health category
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    Minimum typical age
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    Number of records
    Number of records for unique individuals.
    Personal data
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    URI http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/CollectionEvents/name=EGAS00001005376%201M-scBloodNL&resource=EGAS00001005376