EGAS00001003735 Gene expression by RNAseq in bronchial biopsies of asthmatics, asthma in remission and healthy subjects

The study focus was differential expression in bronchial biopsies between persistent asthma, asthma in remission and healthy controls using RNAseq. There were 184 samples that passed QC. RNA samples were processed using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA), using an automated procedure in a Caliper Sciclone NGS Workstation (PerkinElmer, Waltham, MA). In this procedure, all cytoplasmic and mitochondria rRNA was removed (RiboZero Gold kit). The obtained cDNA fragment libraries were loaded in pools of multiple samples unto an Illumina HiSeq2500 sequencer using default parameters for paired-end sequencing (2 × 100 bp). Data are available as 221 pairs of FASTQ-files. Note that several samples are associated with multiple sequence runs.

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Title	EGAS00001003735 Gene expression by RNAseq in bronchial biopsies of asthmatics, asthma in remission and healthy subjects
Description	The study focus was differential expression in bronchial biopsies between persistent asthma, asthma in remission and healthy controls using RNAseq. There were 184 samples that passed QC. RNA samples were processed using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA), using an automated procedure in a Caliper Sciclone NGS Workstation (PerkinElmer, Waltham, MA). In this procedure, all cytoplasmic and mitochondria rRNA was removed (RiboZero Gold kit). The obtained cDNA fragment libraries were loaded in pools of multiple samples unto an Illumina HiSeq2500 sequencer using default parameters for paired-end sequencing (2 × 100 bp). Data are available as 221 pairs of FASTQ-files. Note that several samples are associated with multiple sequence runs.
Keywords	atshma in remission bronchial biopsies healthy controls asthma RNAseq
Contact points	Contact point 1 URI http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/Contacts/firstName=Maarten%20van%20den&lastName=Berge&resource=EGAS00001003735 Name Maarten van den Berge Name (translations) Email m.van.den.berge@umcg.nl Identifier URL
Publisher	Publisher 1 URI http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/Agents/id=UMCG&resource=EGAS00001003735 Name University Medical Center Groningen Name (translations) Email researchdatacatalogue@umcg.nl URL https://www.umcg.nl/ Type Identifier https://ror.org/03cv38k47
Creator	Creator 1 URI http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/Agents/id=UMCG&resource=EGAS00001003735 Name University Medical Center Groningen Name (translations) Email researchdatacatalogue@umcg.nl URL https://www.umcg.nl/ Type Identifier https://ror.org/03cv38k47
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Identifier	https://ega-archive.org/datasets/EGAD00001005112
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Access rights	http://publications.europa.eu/resource/authority/access-right/NON_PUBLIC
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Theme	http://publications.europa.eu/resource/authority/data-theme/HEAL
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Applicable legislation	http://data.europa.eu/eli/reg/2022/868/oj
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URI	http://umcgresearchdatacatalogue.nl/catalogue_rdf/api/rdf/CollectionEvents/name=EGAS00001003735%20Gene%20expression%20by%20RNAseq%20in%20bronchial%20biopsies%20of%20asthmatics%2C%20asthma%20in%20remission%20and%20healthy%20subjects&resource=EGAS00001003735